Volume 174, Issue 15 p. 380-380
Research

Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples

W. H. M. van der Poel PhD, DVM

Corresponding Author

W. H. M. van der Poel PhD, DVM

Central Veterinary Institute of Wageningen UR (CVI), The Netherlands

E-mail for correspondence: [email protected]Search for more papers by this author
B. Cay PhD, DVM

B. Cay PhD, DVM

Veterinary and Agrochemical Research Centre (VAR-CODA), Belgium

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S. Zientara PhD, DVM

S. Zientara PhD, DVM

Agency for Food, Environmental and Occupational Health & Safety (ANSES), France

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F. Steinbach PhD, MRCVS

F. Steinbach PhD, MRCVS

Dept. of Virology, Animal Health and Veterinary Laboratories Agency (AHVLA), UK

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J. F. Valarcher PhD, DVM

J. F. Valarcher PhD, DVM

National Veterinary Institute (SVA), Sweden

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A. Bøtner PhD, DVM

A. Bøtner PhD, DVM

National Veterinary Institute (DTU-VET), Denmark

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M. H. Mars PhD, DVM

M. H. Mars PhD, DVM

Animal Health Service (GD), The Netherlands

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R. Hakze-van der Honing

R. Hakze-van der Honing

Central Veterinary Institute of Wageningen UR (CVI), The Netherlands

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H. Schirrmeier

H. Schirrmeier

Friedrich Loeffler Institut (FLI), Germany

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M. Beer PhD, DVM

M. Beer PhD, DVM

Friedrich Loeffler Institut (FLI), Germany

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First published: 12 April 2014
Citations: 21

Provenance: not commissioned; externally peer reviewed

Abstract

Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories. All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.